According to the World Health Organization, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cancers are caused by preventable damage to DNA by UV light. In this experiment, your students will expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.
Kit includes: instructions, standard DNA fragments, plasmid DNA, gel loading solution, agarose, electrophoresis buffer, 1 ml pipette, microtubes, 100 ml graduated cylinder, DNA stain.
All you need: electrophoresis tank, power supply, adjustable micropipette and tips.
For 10 groups of students.
Time required: 2 hours.