Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format.
In this advanced experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
For 25 students.
Time required: 3 hours and 45 minutes.
Kit includes: instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.
All you need: DNA electrophoresis equipment, thermal cycler, 5-50 µl micropipettes, blue light transilluminator, water bath, microcentrifuge, microwave or hot plate, & isopropanol.