The objective of this experiment is to investigate the genetic basis of beak size variation in Galapagos finches before and after a drought event using Restriction Fragment Length Polymorphism (RFLP) analysis. By analyzing DNA samples collected from finches with different beak sizes, students determine the association between specific genetic variations and variations in beak size. This allows students to explore the role of natural selection in shaping beak morphology, providing insights about evolution at the genetic level.
• Introduces PCR-RFLP analysis to analyze specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Meets NGSS HS DCI LS1.A, LS3.A and LS3.B, through the discussion of the structure and function of DNA and heredity and variance of traits.
Kit includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain
All you need: DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Time required: Complete in 45 minutes
For 8 Gels.