In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. In this kit, students will type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.
For 25 students.
Time required: extraction 50 minutes, PCR set up 10 minutes, PCR 90 minutes, electrophoresis 60 minutes, staining 5 minutes to overnight.
Kit includes: Instructions, PCR EdvoBeads™ PLUS, D1S80 LyphoPrimer™ mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR®Safe Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.
All you need: micropipettes to measure between 5 and 50µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.