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Edvotek 332 Mitochondrial DNA Analysis Using PCR

332 Mitochondrial DNA Analysis Using PCR - Edvotek

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The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA.

For 25 students.

Time required: extraction 50 minutes, PCR set up 10 minutes, PCR 90 minutes, electrophoresis 60 minutes, staining 5 minutes to overnight.

Kit includes: Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR®Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.

All you need: micropipettes to measure between 5 and 50µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.