In this experiment, students will prepare a seed extract from Jack Bean Meal, fractionate the extract by affinity chromatography, and elute the bound glucose binding protein. The presence of biological activity is determined by an immunoblot enzyme assay.
Kit includes: instructions, affinity gel, jack bean meal, various solutions and buffers, petri plates, columns with tips, conical tubes, membranes, and transfer pipettes.
All you need: clinical centrifuge, vortex or shaking platform, micropipette and tips, ring stands and clamps, lab glassware, pipets & pumps, microtubes, forceps, water.
Time required: 2 hours.
For 10 groups of students.