The Green Fluorescent Protein (GFP), which is responsible for bioluminescence in the jellyfish Aequorea victoria, is used extensively in all areas of science. Many organisms have been transformed with the GFP gene. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP).
In this simple experiment your students will transform bacteria either with GFP, BFP or both!
For 10 groups of students.
Time required: 50 minutes and grow overnight.
Kit includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile piettes and loops.
All you need: waterbath, 37ºC incubation oven, long wave UV lamp.