Explore cutting-edge biotechnology with this hands-on CRISPR-Cas9 simulation. Students will assume the role of plant geneticists working to develop corn crops capable of surviving, and thriving, in a changing environment. This experiment will allow students to develop an understanding of CRISPR-Cas9 applications in the laboratory, cleave DNA, and examine their results after gel electrophoresis.
• Hands-On Crispr Cas 9 Simulation
• Explore this cutting edge technology with minimal equipment and class time requirements
• Create four guide RNAs (gRNAs), then use BLAST to verify and evaluate their specificity
• Test all four gRNAs plus control and analyze results using gel electrophoresis
Kit includes: Instructions, Maize DNA samples, DNA Standard Marker, Simulated sgRNAs and Cas9 enzyme, CRISPR Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, & Microcentrifuge Tubes.
All you need: DNA Electrophoresis setup, Micropipettes: 5-50 µl (Optional), & Microwave or Hot Plate.
Time required: Complete in 1 hour 20 minutes to 1 hour 45 minutes
For 8 Gels