In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.
Kit includes: instructions, ready to load DNA samples, agarose powder, practice gel loading solution, electrophoresis buffer, DNA stain and fine tipped transfer pipettes.
All you need: electrophoresis equipment and micropipettes.
Time required: 45 minutes.
For 8 Gels.