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Edvotek 112 Analysis of Eco RI Cleavage Patterns of Lambda DNA

112 Analysis of Eco RI Cleavage Patterns of Lambda DNA

Vendor
Edvotek
Regular price
£69.00
Sale price
£69.00

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.

Kit includes: instructions, ready to load DNA samples, agarose powder, practice gel loading solution, electrophoresis buffer, DNA stain and fine tipped transfer pipettes.

All you need: electrophoresis equipment and micropipettes.

Time required: 45 minutes.

For 8 Gels.