This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
Kit includes: instructions, ready to load DNA samples, agarose powder, practice gel loading solution, electrophoresis buffer, DNA stain and fine tipped transfer pipettes.
All you need: electrophoresis equipment and micropipettes.
Time required: 45 minutes.
For 8 Gels.