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S-53 Mystery of the Crooked Cell

S-53 Mystery of the Crooked Cell

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This simple kit demonstrates detection of the mutation that causes Sickle Cell Anemia. In this simulation, your students will use electrophoresis to separate dyes that represent patient samples and controls.

• Learn about sickle cell anemia and the mutations that cause it
• Introduce the medical biotechnologies of restriction enzyme digestion and electrophoresis
• Perform agarose gel electrophoresis to separate different sized dye molecules
• Load, run, and analyze simulated patient samples and controls to diagnose this condition

For 10 groups of students (10 gels).

Time required: 45 minutes.

Kit includes: instructions, dye samples, agarose, electrophoresis buffer, practice gel loading solution, disposable pipettes.

All you need: electrophoresis equipment and fixed volume or adjustable micropipettes that measure 40µl.