Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format.
In this advanced Edvotek experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
For 25 students.
Time required: 3 hours and 45 minutes.
Kit includes: instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.
All you need: DNA electrophoresis equipment, thermal cycler, 5-50 µl micropipettes, blue light transilluminator, water bath, microcentrifuge, microwave or hot plate, & isopropanol.