In this experiment, students will explore the genetic diversity of fish species in a simulated DNA barcoding experiment. Students will extract DNA from simulated fish samples and use PCR to amplify three gene regions that are conserved between species.
• Learn how to extract DNA from simulated fish samples and amplify specific gene regions using PCR.
• Understand how conserved gene regions can be used to identify species and study genetic diversity.
• Explore DNA barcoding, a critical tool in species identification and conservation biology.
Kit includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, Red Snapper Template, DNA Templates #1-3, TE Buffer, Universal DNA Buffer, Proteinase K, Rice Paper for Simulated Fish Samples, Ultra-Spec Agarose™, 50x Electrophoresis Buffer, FlashBlue™ DNA Stain, SYBR® Safe, 1.5mL Microcentrifuge Tubes, 1.5mL Screw-cap Tubes, 0.2mL PCR Tubes, Petri Plates.
All you need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlue™ stain), Microcentrifuge & Microwave or Hot plate.
Time required: Set Up - 30-45 minutes, PCR - 2 hours or overnight, electrophoresis - 45 minutes.
For 24 reactions, or 6 groups of 4 students.