Your students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.
For 25 students.
Time required: extraction 50 minutes, PCR set up 10 minutes, PCR 90 minutes, electrophoresis 60 minutes, staining 5 minutes to overnight.
Kit includes: Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR®Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.
All you need: micropipettes to measure between 5 and 50µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.