Wastewater testing is a public health tool that helps scientists track specific diseases in a community. In this experiment, students use electrophoresis to test simulated water samples for bacteria and viruses. By learning how wastewater monitoring works, students see how biotechnology helps protect public health.
• Students simulate real-world disease surveillance by analyzing wastewater for Norovirus and E. coli using gel electrophoresis.
• Hands-on use of core biotech tools to detect and identify pathogens in simulated water samples.
• Demonstrates how wastewater testing gives early warnings of outbreaks and informs public health responses.
• This project was made possible by a Science Education Partnership Award (SEPA), Grant Number 2R44GM143977-02, from the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH).
Kit includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain.
All you need: DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Time required: 45 minutes.
For 8 Gels