Due to the worldwide spread of the respiratory disease COVID-19, scientists developed diagnostic tests in order to identify and monitor the disease. In this simulated medical test, students will perform the ELISA to detect the presence of COVID-19 antibodies in simulated patient samples.
**Please note, this experiment is a simulation and intended solely for educational purposes. There is no virus or human blood products included with this experiment. The experiment will not detect SARS-CoV-2 antibodies in human blood samples.**
For 10 groups of students.
Time required: 1 hour.
Kit includes: 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary & secondary antibodies (lyophilized), ABTS substrate (lyophilized), ABTS reaction buffer, microtiter plates, microcentrifuge tubes, & transfer pipets.
All you need: Distilled or deionized water, beakers, pipet pump or bulb, lab glassware, disposable lab gloves, & safety goggles. Recommended: automatic micropipettes (0-50 µl, 100-1000 µl) and tips.