In this experiment, students will gain an understanding of the traditional three-step Polymerase Chain Reaction (PCR). Using PCR and Agarose Gel Electrophoresis, they will analyze a small section of Lambda DNA in a time-saving two-step process.
For 10 student groups.
Time required: set up 10 minutes, PCR 30 minutes, electrophoresis 30 minutes, staining 5 minutes to overnight.
Kit includes: instructions, PCR beads, DNA template, primers, DNA ladder, ultra pure water, agarose, loading dye, electrophoresis buffer, gel stain.
All you need: micropipettes to measure between 5 - 50µl, tips, thermal cycler, electrophoresis tank and power supply.