In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
For 10 groups of students.
Time required: 50 minutes and grow overnight.
Kit includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile piettes and loops.
All you need: waterbath, 37ºC incubation oven, long wave UV lamp.