In this experiment, students will conduct a DNA fingerprinting exercise on simulated patient samples to determine if family members are heterozygous or homozygous for Huntington's Disease. Students will then analyse the amplified DNA segments by agarose gel electrophoresis.
For 5 complete sets of reactions.
Time required: PCR set up 10 minutes, PCR 90 minutes, electrophoresis 60 minutes, staining 5 minutes to overnight.
Kit includes: instructions, PCR EdvoBeads™, LyphoPrimer™ and LyphoTemplate™ DNA, DNA samples, TE buffer, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentifuge tubes, 0.2 ml PCR tubes, and wax beads.
All you need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, microcentrifuge, UV transilluminator or UV photodocumentation system, UV safety goggles, white light visualisation system (optional if staining with FlashBlue™), automatic micropipettes and tips, microwave or hot plate, 250 ml flasks or beakers, hot gloves, and disposable lab gloves.
Storage: Some components require freezer storage.