Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this Edvotek experiment, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis.
For 10 lab groups.
Time required: extraction 55 minutes, PCR set up 10 minutes, PCR 120 minutes, electrophoresis 60 minutes, staining 5 minutes.
Kit includes: Instructions, PCR EdvoBeads™, Universal DNA Buffer, TE Buffer, LyphoPrimer™ Mix, GMO Positive LyphoControl™, NaCl Solution, DNA Extraction Buffer, 100 base pair ladder, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, 0.2 mL PCR Tubes, Plastic Micropestles, Transfer Pipets, and Snap-top Microcentrifuge Tubes..
All you need: DNA Electrophoresis equipment, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, Isopropanol, & 70% Ethanol.