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908 Introduction to Plant Cell Culture

Introduction to Plant Cell Culture - Edvotek 908

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Genetic modification of plants is a highly controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant "stem cells".

In this Edvotek experiment, your students will establish cell cultures of African Violets from leaves. They will then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

For 10 student groups.

Time required: 1 hour plus several weeks for growth.

Kit includes: instructions, shoot initiation and elongation growth medium, Tween, Petri dishes, growth containers, peat pellets.

All you need: A healthy African Violet (Saintpaulia ionantha), microwave or hot plate.