Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor for both conditions. The genetic disease familial hypercholesterolemia (FH) causes an increase in blood levels of the "bad" form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death.
This experiment includes reagents for the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.
For 10 student groups.
Time required: 2 hours and 15 minutes.
Kit includes: instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, acidification solution, color enhancer & color developer, agarose, electrophoresis buffer, InstaStain ethidium bromide.
All you need: horizontal gel electrophoresis apparatus, power supply, adjustable micropipette with tips, balance, microwave oven or hot plate, incubation oven or waterbath, spectrophotometer and cuvettes, UV transilluminator, pipette pumps or bulbs, lab glassware, large weigh boats.