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Edvotek 243 Ion Exchange Chromatography

Ion Exchange Chromatography - Edvotek 243

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Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically.

In this Edvotek experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

For 10 student groups.

Time required: 60-90 minutes.

Kit includes: instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns.

All you need: spectrophotometer & cuvettes, ring stands and clamps, 5 ml pipettes.