Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.
For 10 student groups.
Time required: 60-90 minutes.
Kit includes: instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns.
All you need: spectrophotometer & cuvettes, ring stands and clamps, 5 ml pipettes.