The Green Fluorescent Protein (GFP), which is responsible for bioluminescence in the jellyfish Aequorea victoria, is used extensively in all areas of science. Many organisms have been transformed with the GFP gene. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP).
In this simple Edvotek experiment your students will transform bacteria either with GFP, BFP or both!
For 10 groups of students.
Time required: 50 minutes and grow overnight.
Refill pack includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth. No plastics are included.
All you need: petri dishes, sterile pipettes and loops, waterbath, 37ºC incubation oven, long wave UV lamp.