The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size).
In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed using agarose gel electrophoresis.
Kit includes: instructions, DNA samples, DNA ladder, Dryzymes (Eco RI) agarose, practice gel loading solution, loading dye, electrophoresis buffer, microtipped transfer pipettes, gel stain.
All you need: micropipettes to measure between 5 & 50 µl, tips, waterbath, electrophoresis tank and power supply.
Time required: 90 minutes.
For 10 restriction digestions and 5 gels.