In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.
For 6 restriction digestions.
Time required: 1 hour 20 minutes to 1 hour 45 minutes.
Kit includes: instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps
All you need: DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.