Gel filtration chromatography separates molecules on the basis of size and shape. This experiment provides a LyphoSample mixture of RNA and DNA that is separated on a gel exclusion column. The purified fractions of DNA and RNA are analyzed by agarose gel electrophoresis.
For 5 separations and 5 gels.
Time required: 90 minutes.
Kit includes: instructions, DNA/RNA LyphoSample™, Dry matrix, elution buffer, chromatography columns, agarose, gel loading solution, buffer, InstaStain® Blue and FlashBlue™ stain.
All you need: electrophoresis apparatus, power supply, ring stands with clamps, automatic micropipet with tips, microwave or hot plate, pipet pumps or bulbs, 5 or 10 ml pipets, balance, lab glassware, weigh boats, white light visualization system, photodocumentation system (optional), distilled water.