Gram staining is a quick, effective, and medically relevant identification test that has become one of the most essential tools in bacterial classification. In this experiment, students will use this staining technique to examine the size, shape, arrangement, and gram status (+/-) of Escherichia coli, Bacillus subtilis, and Micrococcus luteus.
Kit includes: Instructions, BactoBeads™, bottle of ReadyPour™ Luria Broth Agar, large petri plates, 10 mL sterile pipet, inoculating loops, toothpicks, transfer pipets, microscope slides, Gram's Crystal Violet, Gram's Iodine, Gram's Safranin, sterile water, microcentrifuge tubes.
All you need: Microscopes, microscope cover slips and oil (if required), microwave, pipet pump, incubator (recommended), timers, slide holders or clothespins, bunsen burner or alcohol lamp, lab marker, beakers, small staining tray, squeeze bottles (recommended), paper towels or blotting paper, 95% ethanol, distilled water, gloves.
Time required: Complete in 90 minutes.
For 8 Lab Groups.