Using prestained Edvotek LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained Proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
Kit includes: instructions, denatured LyphoProtein samples, standard protein markers, gel loading solution, buffer, Protein Plus stain & Protein InstaStain.
All you need: 3 polyacrylamide gels (12%), MV10 vertical gel electrophoresis apparatus, power supply, white light box, 5-50 ul adjustable or 20 ul fixed volume micropipette, fine tips, methanol, glacial acetic acid.
For 6 student groups sharing 3 gels.
- Electrophoresis 60 minutes
- Staining 20 minutes
- Destaining 2 hours