In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.
Kit includes: instructions & Ready-to-Load™ DNA samples for 24 gels.
All you need: electrophoresis apparatus, power supply, agarose powder, buffer solution, automatic micropipet and tips, balance, microwave or hot plate, visualization system.
Time required: 50 minutes.
For 24 gels.