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113 Principles of Thin Layer Chromatography

113 Principles of Thin Layer Chromatography

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This experiment introduces chromatographic theory and methods of thin layer chromatography. A mixture of dyes are separated on a cellulose-based TLC plate using two different solvent systems.

For 8 student groups.

Time required: spotting plates 20 minutes, TLC separation 5 minutes.

Kit includes: instructions, samples, reagents and solvents, cellulose thin layer plate, 5 ul glass capillary pipettes.

All you need: 250 ml beakers, metric rulers, pipet pump, 5 or 10 ml pipets, distilled water.