This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
Kit includes: instructions & Ready-to-Load™ dye samples for 24 gels.
All you need: electrophoresis apparatus, power supply, agarose powder, buffer solution, automatic micropipet and tips, balance, microwave or hot plate, visualization system.
Time required: 45 minutes.
For 24 Gels.