The objective of this experiment is for students to understand the theory and application of western blotting. Students will perform a western blot to determine simulated clinical trial participants' risk of developing Alzheimer's Disease.
For 6 student groups with 2 groups per polyacrylamide gel.
Time required: Electrophoresis 1 hour, staining 1-3 hours or overnight.
Kit includes: instructions, pre-stained LyphoProtein™ gel marker, pre-stained LyphoProteins™, immunochemical and blotting reagents, 10x Tris-glycine-SDS buffer (chamber buffer), 10x Tris-glycine powdered buffer (transfer buffer), practice gel loading solution, nylon membrane, filter paper, and large filter paper.
All you need: 12% denaturing polyacrylamide gels (3), vertical gel electrophoresis apparatus, power supply, shaker platform (optional), automatic pipets with tips, microtest tubes, beakers, transfer pipets, graduated cylinders, plastic wrap, scissors, trays or containers, forceps, several packs of paper towels, latex or vinyl lab gloves, safety goggles, methanol, and distilled water.