The brain is an incredibly complex organ and is responsible for regulating almost everything within our body. It allows us to form complex thoughts, read, write, move, breathe, play sports, and listen to music. It does this through a network of cells working together to function.
The objective of the experiment is for students to examine the differences between cell types in the brain based on their profiles of proteins.
For 6 Groups Sharing 3 Polyacrylamide Gels.
Time required: Electrophoresis 1 hour, staining 1-3 hours or overnight.
Kit includes: pre-stained protein standard markers (lyophilized), protein samples (lyophilized), 10x Tris-Glycine-SDS buffer (chamber buffer), Protein InstaStain®, practice gel loading solution, transfer pipettes.
All you need: 12% denaturing polyacrylamide gels (3), vertical gel electrophoresis apparatus, power supply, shaker platform (optional), adjustable pipettes with tips, glacial acetic acid, methanol, beakers, transfer pipettes, foil, trays or containers, spatula, latex or vinyl lab gloves, safety goggles, distilled water.