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110 Molecular Weight Determination of Proteins (Agarose-based)

110 Molecular Weight Determination of Proteins (Agarose-based) - Edvotek

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Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and "unknowns" are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is Edvotek´s formulation of protein grade agarose, which provides an alternative to the use of polyacrylamide gels.

For 6 groups of students.

Time required: gel preparation 40 minutes, electrophoresis 60 minutes.

Kit includes: instructions, prestained LyphoProtein™ samples, gel loading solution, agarose, electrophoresis buffer, stain, SDS solution.

All you need: horizontal electrophoresis apparatus, power supply, white light visualization system, automatic micropipet with tips, microwave oven or hot plate, waterbath, metric rulers, lab glassware, methanol, glacial acetic acid, distilled or deionized water.