Can your class save the peas? In this scenario, they will test 5 different fields of peas to see if they are infected with a devastating plant pathogen using PCR.
Tests that look for the presence or absence of a DNA band must have an internal control to check the test has worked. A test for a constantly expressed “housekeeping” gene must be included.
Show how PCR can detect pathogens, electrophoresis and the importance of using a control in experiments.
Developed in partnership with Dr Phil Smith, Teacher Scientist Network, Norwich, UK
Kit contents: instructions, dye samples, agarose, electrophoresis buffer.
Refill pack also available: Click for details.
All you need: electrophoresis tank, power supply, fixed volume or adjustable 40ul micropipette and tips.
Time required: 45 minutes.
For 12 gels.