Quantitative PCR (qPCR also known as real-time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technology has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics.
For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands-on learning, and an essential skill for future research work.
In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis.
*** Please note this kit must be used with a Real Time Thermal Cycler ***
Kit includes: instructions, qPCR Master Mix with Fluorescent Dye, Primer Mix, DNA Template, Experimental DNA Samples, Ultra-Pure Water, DNA Standard Marker, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10x Gel Loading Solution, SYBR® Safe Stain, Microcentrifuge & qPCR Tubes.
All you need: qPCR Thermal Cycler, Microcentrifuge, Micropipettes: 5-50 μl, Horizontal Gel Electrophoresis Apparatus, DC Power Supply, UV Transilluminator or White Light Box, Microwave or Hot Plate, Pipet Pump, Balance, Hot Gloves, Laboratory Gloves, Beaker, Distilled Water, Ice.
Time required: QPCR setup - 45 minutes, QPCR - 90 minutes, electrophoresis - 60 minutes, staining - 5 minutes to overnight, analysis of results 45 minutes.
For 4 groups of students