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Edvotek 212 Cleavage of Lambda DNA with Eco RI Restriction Enzyme

212 Cleavage of Lambda DNA with Eco RI Restriction Enzyme

Regular price
£85.00
Sale price
£85.00

The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size).

In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed using agarose gel electrophoresis.

Kit includes: instructions, DNA samples, DNA ladder, Dryzymes (Eco RI) agarose, practice gel loading solution, loading dye, electrophoresis buffer, microtipped transfer pipettes, gel stain.

All you need: micropipettes to measure between 5 & 50 µl, tips, waterbath, electrophoresis tank and power supply.

Time required: 90 minutes.

For 10 restriction digestions and 5 gels.