The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size).
In this Edvotek experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed using agarose gel electrophoresis.
Kit includes: instructions, DNA samples, DNA ladder, Dryzymes (Eco RI) agarose, practice gel loading solution, loading dye, electrophoresis buffer, microtipped transfer pipettes, gel stain.
All you need: micropipettes to measure between 5 & 50 µl, tips, waterbath, electrophoresis tank and power supply.
Time required: 90 minutes.
For 10 restriction digestions and 5 gels.