This experiment introduces your students to Southern blotting as a tool for "DNA Fingerprinting" in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.
For 5 groups of students.
Time required: electrophoresis 45 minutes, blotting overnight and staining & destaining 10 minutes.
Kit includes: instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.
All you need: electrophoresis apparatus, power supply, 65º C Waterbath, DNA visualization system, staining net & tray, automatic micropipets, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps.