Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experiments. In this Edvotek experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
For up to 20 plasmid isolations for up to 12 (7x7cm) gels.
Time required: 1 hour 45 minutes.
Kit includes: Instructions, Plasmid LyphoCells™, Tris Buffer Concentrate, Sodium Hydroxide Solution, SDS Solution, Resuspension Buffer, Potassium Acetate Solution, RNase Solution, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, & Microcentrifuge Tubes.
All you need: DNA electrophoresis equipment, Micropipettes: 5-50 µl, Microcentrifuges, White Light Box, Microwave or Hot Plate, Isopropanol, & 70% Ethanol.